| Analysis Note | The biological activity is measured by its ability to inhibit sonic hedgehog (Shh) induction of alkaline phosphatase production in C3H10T1/2 fibroblasts. |
| Physical form | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin. |
| Biochem/physiol Actions | Hedgehog Interacting Protein (Hip) is a novel component in the vertebrate signaling pathway, which has ability to bind Hedgehog proteins. Hip encodes a membrane glycoprotein that binds to all three mammalian Hedgehog proteins with an affinity comparable to that of Ptc-1. Hip, like Ptc-1, is a general transcriptional target of Hedgehog signalling. Overexpression of Hip in cartilage where Indian hedgehog (Ihh) controls growth leads to a shortened skeleton that resembles that seen when Ihh function is lost (B. St-Jacques, M. Hammerschmidt & A.P.M., in preparation). bHip attenuates Hedgehog signalling as a result of binding to Hedgehog proteins: a negative regulatory feedback loop established in this way could thus modulate the responses to any Hedgehog signal. |
| Specifications | Based on N-terminal sequencing, this preparation contains a mixture of at least two peptides that have Lys 24 and Asp 52, respectively, at the N-terminus. The two peptides have calculated molecular masses of ~76 kDa and 73 kDa, respectively. Due to glycosylation and C-terminal proteolytic processing, recombinant mouse Hip migrates with apparent molecular masses of 80-90 kDa and 58-64 kDa, respectively, in SDS-PAGE under reducing conditions. |
